Biological Imaging
Confocal Microscope System
Introduction
The Laser Scanning Confocal Microscope (LSCM) has the ability to view fluorescent specimens for biological and biomedical applications amongst others. Samples are treated with fluorescent labels to selectively highlight regions of interest and solid-state lasers are used to irradiate and excite specimens with the ability to view samples at an axial and lateral resolution of down to 200nm. Due to the planar imaging capabilities of the technique, 3D images can be constructed giving the user a better understanding of the sample being viewed.
Applications
The LSCM has the capabilities to scan single optical sections as well as a sequence of sections to view different levels and thus attain a three-dimensional image. With the ability to image fixed and living cells, a time-lapse study with improved resolutions is also easily achieved. The LSCM at MCN can also be
integrated with the JPK Atomic Force Microscope to combine extremely high resolution surface imaging with confocal imaging.
Equipment
The Nikon A1Rsi Confocal Microscope is attached to an inverted microscope, where a laser is combined with photomultiplier tube detector and images are collected and displayed on a computer. Various software can be used to process the data.
Atomic Force Microscopy
Introduction
The Nanowizard II Atomic Force Microscope (AFM) is designed for imaging fixed and live cells in biological/ chemical fluids or air. Custom designed cantilevers are acquired for imaging and manipulation of samples where force measurements of samples can also be attained. The system features stand-alone tip scanning allowing for flexibility in applications whilst maintaining high resolutions.
Applications
The AFM can be used to understand surface chemistry of cells and measure force spectroscopy of surfaces. The JPK Atomic Force Microscope can also be integrated with the LSCM at MCN.
Equipment
The Atomic Force Microscope is attached to an inverted microscope, where images are collected and displayed on a computer. Various software can be used to process the data.
Contacts
For more information about the Atomic Force Microscope at MCN or enquiries about access, please contact.
Ms Varsha Lal
Varsha.lal@monash.edu
990229657
