Microscopy
Ellipsometer
Optical Profiler
Profilometer
Scanning Electron Microscope (SEM)
Total internal reflection fluorescence
Atomic Force Microscope (AFM)
Introduction
The Nanowizard II Atomic Force Microscope (AFM) is designed for imaging fixed and live cells in biological/ chemical fluids or air. Custom designed cantilevers are required for imaging and manipulation of samples where force measurements of samples can also be attained. The system features stand-alone tip scanning allowing for flexibility in applications whilst maintaining high resolutions.
Applications
The AFM can be used to understand surface chemistry of cells and measure force spectroscopy of surfaces. The JPK Atomic Force Microscope can also be integrated with the LSCM at MCN.
Projects
Characterisation of gold nanoparticle surface assemblies
Industry looks to partner with MCN in R&D
Measurement and prediction of vulnerable plaque formation and rupture
Controlling collisions between drops and bubbles using nanofabrication methods
Equipment
The Atomic Force Microscope is attached to an inverted microscope, where images are collected and displayed on a computer. Various software can be used to process the data.
Contacts
For more information about the Atomic Force Microscope at MCN or enquiries about access, please contact.
Ms Varsha Lal
990229657
Confocal Microscopy
Introduction
With the use of Laser Scanning Confocal Microscopy (LSCM), the ability to view fluorescent specimens for biological and biomedical applications has largely increased over conventional optical microscopy. LSCM incorporates solid-state lasers to irradiate and excite specimen at specific wavelengths, with the ability to view samples at an axial and lateral resolution of 200nm.
Applications
The LSCM has the capabilities to scan single optical sections as well as a sequence of sections to view different levels and thus attain a three-dimensional image. With the ability to image fixed and living cells, a time-lapse study with improved resolutions is also easily achieved. The LSCM at MCN can also be integrated with the JPK Atomic Force Microscope.
Projects
Nanoparticle delivery into cells using engineered pore forming proteins
Equipment
The Nikon A1Rsi Confocal Microscope is attached to an inverted microscope, where a laser is combined with photomultiplier tube detector and images are collected and displayed on a computer. Various software can be used to process the data.
Contacts
For more information about the Confocal microscope at MCN or enquiries about access, please contact.
Ms Varsha Lal
990229657