Nanofabulous Seminar: Advancing Confocal Super-Resolution Imaging: From STED to FSTM
Stimulated Emission Depletion (STED) super-resolution imaging typically requires high-power depletion lasers, which can cause significant sample damage and phototoxicity. To address this challenge, we developed novel strategies combining advanced optical methods and fluorescent probes to lower depletion light power while enhancing imaging performance. This enables applications in live-cell imaging and even in vivo imaging. Additionally, leveraging time-resolved detection and computational imaging techniques, we introduced a phase analysis-based spatiotemporal fluorescence modulation strategy (FSTM). This approach allows us to extract precise photon information from both temporal and spatial dimensions. Using a single-pulse laser, we can also achieve multicolor super-resolution imaging of live cells, as well as super-resolution fluorescence lifetime imaging.
Prof Junle Qu
Center for Biomedical Photonics
College of Physics and Optoelectronic Engineering
Shenzhen University, China
11:00am, 06/05/2025
Melbourne Centre for Nanofabrication
151 Wellington Road, Clayton, 3168
Zoom link: click here
Meeting ID: 872 6697 4387 passcode: 184147
